시퀀서 / 시퀀서 장비 Throughput 비교 (NextSeq, MiSeq, HiSeq)

일루미나 시퀀서 장비의 Throughput 비교

http://nextgenseek.com/2014/01/how-does-nextseq-500-compare-with-miseq-and-hiseq/

Whole-Genome Sequencing

How many large whole genome sequencing experiments (human genome at 30X coverage) in a single run?

• MiSeq:  Not applicable
• NextSeq 500: One
• HiSeq 2500: 1 – 10

How many small whole genome sequencing experiments (30X or greater coverage of a 5 Mb microbial genome like E. Coli.) in a single run?

• MiSeq:  1 – 96
• NextSeq 500: 120-792
• HiSeq 2500: 96-6660

Exome Sequencing

How many exome sequencing experiments (4 Gb per sample at 2×75 bp or 5 Gb at 2×100 read length) in a single run?

• MiSeq:  1
• NextSeq 500: 1 – 12
• HiSeq 2500: 12 – 160

RNA Sequencing

How many RNA profiling experiments (10M reads per sample) in a single run?

• MiSeq:  1 – 2
• NextSeq 500: 12 – 36
• HiSeq 2500: 24 – 396

How many Transcriptome Sequencing experiments (40M reads per sample) in a single run?

• MiSeq:  Not applicable
• NextSeq 500: 3 -10
• HiSeq 2500: 8 – 96

How many small RNA sequencing experiments (5M reads per sample) in a single run?

• MiSeq:  1 – 5
• NextSeq 500: 25 – 80
• HiSeq 2500: 60 – 792

Chip-Seq

How many Chip-Seq experiments (15M reads per sample for identification of transcription factor binding) in a single run?

• MiSeq:  1
• NextSeq 500: 8 – 24
• HiSeq 2500: 20 – 264